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KMID : 1041220070490010008
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Journal of Sericultural and Entomological Science
2007 Volume.49 No. 1 p.8 ~ p.13
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Structural Analysis of Natural Indigo Colorants Extracted from Polygonum tinctorium
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Chung In-Mo
Lee Kwang-Gill
Sung Gyoo-Byung
Kim Hyun-Bok
Nam Sung-Hee
Hong In-Pyo
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Abstract
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Natural indigo colorants were prepared by extraction of Polygonum tinctorium which was harvested just in the blooming season(in the late of July). The components were analyzed by TLC and HPLC, and its structures were analyzed by FT-IR, EI-mass. The results obtained are summarized as follows; The natural indigo powder was dissolved in DMSO and developed in eluent, (8.5:1.5 v/v) by means of TLC for its quality analysis. It was segregated into indirubin as a red colour and indigo as a blue colour. In case of HPLC analysis,. FT-IR spectrum of indirubin showed a peak for NH residue between 3200 and . spectrum for indigo displayed AA’BB’ spin system caused by indole structure between 6.5 and 7.7ppm of H4, 5, 6 and 7, and -NH proton for indirubin showed an singlet between 10.88 and 11.0ppm. EI-mass spectrum of indigo and indirubin both disclosed their molecular size as 262 and it implies that these two substances are isomer.
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KEYWORD
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Indirubin, TLC, HPLC, Isomer
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